Clark School Press Release Story

Stopping Bacterial Infections Without Antibiotics

Clark School's Nanofactories Could Be Next Big Step in Antimicrobial Treatment

FOR IMMEDIATE RELEASE  January 27, 2010

CONTACT:
Faye Levine
301-405-0379
flevine@umd.edu

COLLEGE PARK, Md.-New research at the A. James Clark School of Engineering could prevent bacterial infections using tiny biochemical machines--nanofactories--that can confuse bacteria and stop them from spreading, without the use of antibiotics.

A paper about the research is featured in the current issue of Nature Nanotechnology. "Engineered biological nanofactories trigger quorum sensing response in targeted bacteria," was authored by Clark School alumnus Rohan Fernandes (Ph.D. '08, bioengineering), graduate student Varnika Roy (molecular and cell biology), graduate student Hsuan-Chen Wu (bioengineering), and their advisor, William Bentley (professor and chair, Fischell Department of Bioengineering).

The group's work is an update on their original nanofactories, first developed in 2007. Those nanofactories made use of tiny magnetic bits to guide them to the infection site.

"This is a completely new, all-biological version," Bentley says. "The new nanofactories are self-guided and targeted. We've demonstrated for the first time that they're capable of finding a specific kind of bacterium and inducing it to communicate, a much finer level of automation and control."

The new nanofactories can tell the difference between bad (pathogenic) and good bacteria. For instance, our digestive tracts contain a certain level of good bacteria to help us digest food. The new nanofactories could target just the bad bacteria, without disrupting the levels of good bacteria in the digestive tract (a common side effect of many antibiotics). Nanofactories target the bacteria directly rather than traveling throughout the body, another advantage over traditional antibiotics.

Bacterial cells talk to each other in a form of cell-to-cell communication known as quorum sensing. When the cells sense that they have reached a certain quantity, an infection could be triggered. The biological nanofactories developed at the Clark School can interrupt this communication, disrupting the actions of the cells and shutting down an infection.

Alternatively, the nanofactories could trick the bacteria into sensing a quorum too early. Doing so would trigger the bacteria to try to form an infection before there are enough bacterial cells to do harm. This would prompt a natural immune system response capable of stopping them without the use of drugs.

Because nanofactories are designed to affect communication instead of trying to kill the bacteria, they could help treat illness in cases where a strain of bacteria has become resistant to antibiotics.

"The work by Dr. Bentley is extremely exciting as he is using the ability of engineering to "build" using nature based components," says Philip Leduc, associate professor in the Departments of Mechanical and Biomedical Engineering and the Lane Center for Computational Biology and Biological Sciences at Carnegie Mellon University. "Understanding the science of cells is wonderful, but then using these components and constructing systems that leverage biological advantages is a huge step forward. His work in this paper uses his synthetic biology approach to build new nanofactories toward new areas of antimicrobials as well as opening new findings in quorum sensing."

The nanofactories' ability to alter cell-to-cell communication isn't limited to fighting infections.

"Quorum sensing and signaling molecules are actually used to accomplish a lot of things," Bentley explains. "Sometimes disease develops because communication is not taking place—a good example is digestive disorders that involve an imbalance of bacteria in the digestive tract. In that case, nanofactories could be used to start or increase communication instead of disrupting it."

More Information: Read the article at Nature Nanotechnology
Visit Professor Bentley's web site
See a research overview at the Biochip Collaborative web site

About the A. James Clark School of Engineering

The Clark School of Engineering, situated on the rolling, 1,500-acre University of Maryland campus in College Park, Md., is one of the premier engineering schools in the U.S., with graduate and undergraduate education programs ranked in or near the Top 20. In 2012, the Clark School was ranked 14th in the world by the Institute of Higher Education and Center for World-Class Universities in its Academic Ranking of World Universities. Three faculty members affiliated with the Clark School were inducted into the National Academy of Engineering in 2010.

The school, which offers 13 graduate programs and 12 undergraduate programs, including degree and certification programs tailored for working professionals, is home to one of the most vibrant research programs in the country. The Clark School garnered research awards of $171 million last year. With emphasis in key areas such as energy, nanotechnology and materials, bioengineering, robotics, communications and networking, life cycle and reliability engineering, project management, intelligent transportation systems and aerospace, the Clark School is leading the way toward the next generations of engineering advances.